Organization of microtubules in centrosome-free cytoplasm.
نویسندگان
چکیده
منابع مشابه
Organization of microtubules in centrosome-free cytoplasm
Many different cell types possess microtubule patterns which appear to be polarized and oriented, in part, by cytoplasmic factors not directly associated with a centrosome. Recently, we demonstrated that cytoplasmic extensions ("arms") of teleost melanophores will reorganize their microtubule population outward from their centers after surgical isolation (McNiven, M. A., M. Wang, and K. R. Port...
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pithelial microtubules (MTs) rely on the cortex and each other to create stable organized patterns, as shown by Reilein et al. (page 845). MTs are normally organized by centrosomes in animal cells. But in polarized epithelial cells, centrosomes are busy building the primary cilium under the apical membrane, leaving basal MTs to fend for themselves. Reilein and colleagues wanted to know how this...
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After enucleation of PK (pig kidney embryo) cells using cytochalasin D centrioles remained approximately in 80% of cytoplasts. Some cytoplasts retained a single centriole. 14-16 h after enucleation large secondary lysosomes and lipid droplets were evident around the centrosome of many cytoplasts. In part of the cytoplasts replicating centrioles were found 16 h after enucleation. Ouabain treatme...
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Translationally Controlled Tumour Protein (TCTP) associates with microtubules (MT), however, the details of this association are unknown. Here we analyze the relationship of TCTP with MTs and centrosomes in Xenopus laevis and mammalian cells using immunofluorescence, tagged TCTP expression and immunoelectron microscopy. We show that TCTP associates both with MTs and centrosomes at spindle poles...
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The capacity of the centrosome to influence the lattice structure of nucleated microtubules was studied in vitro. Brain microtubules self-assembled to give predominantly (98%) 14-protofilament microtubules. However, under exactly the same conditions of assembly they grew off of purified centrosomes from neuroblastoma cells to give mostly (82%) 13protofilament microtubules. Thus, the nucleation ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1988
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.106.5.1593